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Shanghai centrifuge learned cultivation room inside cells, the centrifuge is one of the necessary instruments most commonly used laboratory, to the number of low speed refrigerated centrifuge. Today we talk about with, in the use of cell culture cell passage technology -:
Cell passage way: adherent cells by digestion passage; suspension cell growth by centrifugal separation after passage.
Cell passages:
Article preparation: super clean bench, CO2 incubator, inverted microscope, low speed refrigerated centrifuge, 95% alcohol lamp, PBS, 0.25%trypsin (more common), 10%FBS (DMEM) (as required), Pasteur, 25mm flasks (as required), 75% alcohol cotton ball tank, waste liquid cylinder.