Low speed large capacity centrifuge on the application of low speed refrigerated centrifuge in cell passage in technology

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Low speed large capacity centrifuge on adherent cell passage presents the steps:
The appropriate 75% alcohol cotton ball wiping ultra clean work table, various items placed in sequence, with regular alcohol lamp;
Cultivating box takes out cell, Straw passage gettering cultivation waste liquid bottle of old cultivation;
The appropriate cleaning two times in PBS cells;
The meeting after the CO2 0.5~1ml trypsin, cultivating box or room temperature 25 2~5 minutes to digest;
The naked eye see show needle like white space; or microscope investigation, found that the cytoplasmic retraction, cell gap increased;
The cultivation of a small amount of liquid is directly added serum containing, stop digestion;
The cells from the bottle wall is formed after the cell suspension, according to the requirements in cultivating new parting inoculation bottle in;
The back CO2 cultivating box continuous cultivation.
Low speed large capacity centrifuge on suspension cells passaged steps are introduced:
Direct passage that let the suspension cells gradually deposited at the bottom of the bottle, will wash out of 1/2 ~ 1/3, and then use the straw and percussion composition of cell suspension, then passaged.
Using low speed refrigerated centrifuge centrifuge method: centrifuge speed is 800 ~ 1000rpm, centrifugal 5min, Kami Kiyo, and foster the new solution, and then according to a certain share of isolated. In the selection of low speed refrigerated centrifuge rotor, general equipped with 5ml, 10ml, 15ml capacity based. Another centrifuge occur heat in high-speed running, some cells react or die Mang in after the heat, therefore suggest that we choose the low velocity zone freezing type centrifuge, if cell heat does not occur in response, reduce the cost we can directly choose low-speed centrifugation