Characteristics of the centrifugal separation method of high speed refrigerated centrifuge

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High speed refrigerated centrifuge is usually in the first centrifugal to particle most don't need settling to remove. A large group of local time required to remain in the supernatant. The supernatant was then collected at high speed centrifugation, the desired particle packing down. Centrifugal time to choose properly, so that the majority did not need smaller particles remain in the supernatant. On the pellet and the supernatant obtained can stop further centrifugation, to reach the demand far separation purity. Characteristics of high speed refrigerated centrifuge is simple, but the separation is not high purity. According to the different particles in samples with different size and settling velocity. Steps are as follows: in the centrifuge tube into the density gradient solution, solution density from the top to the bottom of the centrifugal tube gradually increases (positive gradient). The required separation of samples carefully add to the top of the density gradient solution. High speed refrigerated centrifuge understanding to the sample form a negative gradient in the gradient solution appearance. Due to the different size of particles in the centrifugal force, move in the gradient of the speed is not the same, so after a few samples after centrifugation will constitute separate belt. High speed refrigerated centrifuge suggest the need to note: the sample particle density must be greater than at any point in the density gradient liquid injection. Centrifugal process must be in the zone at the bottom of the pipe before the suspension of arrival. Isopycnic centrifugation (isopycnic): according to different density separation of particles. High speed refrigerated centrifuge on centrifugal process, the particles will move to the same density and its central constitute zone. Density gradient is selected to make the gradient range includes everything to be parting particle density. The sample can in the gradient liquid density above average grain or scattered in the density gradient. After centrifugation, the sample particles reach the balance.