Low speed large capacity centrifuge for the working principle of the Biological Sciences

Low speed large capacity centrifuge for the working principle of the Biological Sciences

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Application of Biological Science in low speed large capacity centrifuge used in is not Things seldom seen are strange. thing, centrifugal technology in biological science, especially in biochemistry and molecular biology research, has played an important role, each biochemistry and molecular biology laboratory and all kinds of universities to various types of high-speed centrifuge device. Centrifugal technology is mainly used for various biological sample separation and preparation, biological sample suspensions in high-speed rotation, the centripetal force big, make the tiny particles suspended (organelles, biological macromolecules such as precipitation) at a certain speed to settlement, parting with solution, quality, size and density and settlement speed depends on the particle.
Low speed large capacity centrifuge in order to facilitate the implementation of conversion between the speed and the relative centripetal force, calculation formula of Dole and Cotzias application of RCF, making the speed of "RPM", "RCF" the relative centripetal force and rotating radius "R" the relationship between the three nomograms, schema than formula calculation method Bento (nomogram see Appendix). The conversion, get known in the R on the scale radius and get known in RPM scale on the centrifuge speed, and then draw a straight line between these points, and RCF in the figure on the scale of the intersection is the relative centripetal force, the corresponding numerical. Note that, if known to numerical in RPM scale on the right side, you should read the RCF scale numerical value is right, turn left RPM scale, RCF scale numerical reading should be on the left side of the.
Low speed large capacity centrifuge under normal circumstances, often by low-speed centrifugation speed "RPM" to show, high speed centrifugation is indicated as "g". The calculation of particle relative centripetal force, should pay attention to centrifuge tube and a rotating central shaft interval "R" different, namely the settlement of particles in the centrifugal pipe in different position, the centripetal force is different from. Thus in the report ultracentrifugation conditions, usually with multiple "gravity * g" instead of "RPM" revolutions per minute, because it can reflect the change of particles in centrifugal centripetal force of different location in pipe and its dynamic. The centripetal force of science and technology of data in the literature usually refers to the average value (RCFav), which is the midpoint of the centripetal force, centrifugal tube.