Low speed centrifuge and high speed centrifuge difference

Low speed centrifuge and high speed centrifuge difference

In order to density gradient and the sample zone well, added to the density gradient at the top of the liquid column sample density must be less than the top of the gradient liquid density, that assumption is not so, the sample will be through the gradient method to collapse, until the arrival of density area. Expand the sample in the process layer will be significant, droplets around settlement will constitute the resolution drop zone.
Low speed centrifugal mechanism is provided by a sideline concave and convex gradient gradient, on the contrary, the first from the mixing room flows into the centrifuge tube is the heavy end of the gradient liquid, preparation, light liquid to slowly spread, therefore begged the needle to the corresponding speed up, in most cases, simply be inclined put must view centrifuge tube, the needle is placed in the centrifugal nozzle to roast, the solution slowly flow down along the pipe wall, and gradually spread, add after, then straight put sample adding centrifuge tube.
At this moment, should pay attention to is, the selection of centrifugal tube material best is hydrophilic, hydrophobic centrifuge tube as is available, some salt, so again in the gradient solution, can ensure that the average flow. This kind of low speed centrifuge structure is relatively simple, can be divided into a small bench and floor type two kinds, equipped with a driving motor, speed regulator, timer and other devices, convenient operation. Low speed centrifuge and its speed is generally not more than 4000rpm, centrifuge table high speed maximum speed can reach 18000rpm. so high speed and low speed centrifuge separation are quickly to distinguish the degree, and the application field are not the same.

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