Reference introduces multifunctional mixing technology in uniform instrument

Reference introduces multifunctional mixing technology in uniform instrument
(a) β -Tubulin antibody and make a beautiful picture of the internal WB and immunofluorescenc
Tubulin tubulin, is a kind of skeleton protein cells. Tubulin is divided into alpha, beta, gamma, Delta, epsilon, tubulin, the α -Tubulin and β -Tubulin can form heterodimers of the two polymer, is two Tubulin the main forming microtubules. α -tubulin and β -tubulin molecular weight was 55kDa and 50kDa respectively, the actual test strips are about 55kDa. As a reference, the protein level of β -tubulin usually does not change, it is widely used in Western Blotting whether the sample quantity of reference, were also used to observe the cell immune staining of microtubule struc
Control antibody although more choices, but also need to follow certain principle, application requirements and in accordance with the actual. First of all, we need to consider the size of the target protein, we recommend reference to molecular and detection of the amount of target protein is best is more than 5KD, so you need to select the appropriate reference according to your objective detection of protein molecular weight, the molecular detection of β -Tubulin content is about 55kD, so the internal control is not very suitable for the detection of antibody WB experimental 50kD-60kD target protein in size. In addition, different tissue samples have some differences in the choice of reference when. For example, some special circumstances, such as fatty tissue or cells, the expression of β -Actin is very few, not suitable for referenc
In addition, we also need to consider their own needs, especially the need to experiment on the application. Generally speaking, the more types of application, choice in the use process is larger, and the monoclonal antibody in mice source specificity, stability and potency than polyclonal antibody. In addition, an important aspect is to study the antibody titer of the price, which is equivalent to the actual application of the dilution ratio. A high titer of 100 μ L β -Tubulin antibody (if the dilution ratio WB detection of the 1:5000, the final solution is equivalent to 500ml), β -Tubulin antibody titers to lower than 1ml (if the dilution ratio of WB detection is 1:200, the final solution is equivalent to 2ml) and higher price. So when in the selection of antibodies, not only need to consider the amount of antibody, also need to consider the antibody titers, i.e. different application proposal dilution rat
(two) Actin actin cytoskeleton protein, is a kind of important cell. Actin can be divided into six types, four of them are different muscle tissue specific, and the other two are widely distributed in various tissues, including beta -actin and γ -non-muscle actin. Beta -actin as an internal standard is widely acknowledged, this is the most tissues and cells, which are widely distributed in the cytoplasm of a cell, expression is very rich, the content of total cellular protein 50%. But in some special circumstances, such as fatty tissue or cells, the expression of β -Actin rarely. Beta -actin is composed of 375 amino acids, the molecular weight is about 42-43kD
Control antibody although more choices, but also need to follow certain principle, application requirements and in accordance with the actual. First of all, we need to consider the size of the target protein, we recommend reference to molecular and detection of the target protein was best is more than 5KD, so you need to select the appropriate reference according to your objective detection of protein molecular weight, the molecular detection of β -Actin content is about 42kD, so the control antibody is not very suitable for detection of WB protein in experimental 38kD-48kD size. In addition, although -Actin is one of the most appropriate control antibodies, but under certain conditions, some factors may lead to changes in the sample room of β -Actin content. Of particular note is, in fatty tissues, the expression of β -Actin is very low, is not suitable as a referen
In addition, we also need to consider their own needs, especially the need to experiment on the application. Generally speaking, the more types of application, choice in the use process is larger, and the monoclonal antibody in mice source specificity, stability and potency than polyclonal antibody. In addition, an important aspect is to study the antibody titer of the price, which is equivalent to the actual application of the dilution ratio. A high titer of 100 μ L β -Actin antibody (if the dilution ratio WB detection of the 1:5000, the final solution is equivalent to 500ml), β -Actin antibody titers to lower than 1ml (if the dilution ratio of WB detection is 1:200, the final solution is equivalent to 2ml) and higher price. So when in the selection of antibodies, not only need to consider the amount of antibody, also need to consider the antibody titers, i.e. different application proposal dilution rat