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Introduction and development trend of the centrifuge rotor

Release date:2014-08-20
The rotor is the main parts of the centrifuge. Simply speaking, can be divided into host centrifuge rotor and a detachable (turned) the two part, a multi function host can often be matched with a plurality of the rotor, the rotor by different models to meet different needs to achieve its "multifunctional", which can effectively reduce the purchase cost, but the choose and buy when need to pay attention to the selection of the rotor, convenient disassembly and assembly of centrifuges, centrifuge rotor and spindle general directly with manufacturers in order to reduce the noise, often with host and rotor is quite high, but this is the biggest drawbacks rotor and spindle killed inconvenient disassembly, in addition, with the rotor and the motor has direction sex is very strong, when the direction of the rotor and spindle motors with offset will cause greater noise affects the service life of the machine, it will result in a serious rupture of main shaft, rotor explosion. Like the German sigma centrifuge is the use of expansion sleeve the rotor is fixed on the main shaft, and rotor so that no matter how long can easily, won't appear bite condition. The most commonly used fixed angle rotor comprises a rotor and a horizontal rotor. The rotor is Qi of course to function more complete, so the choice of the rotor is usually chosen according to the capacity and centrifugal common sample conditions, usually horizontal rotor and angular head each one, large capacity (relatively low) of the rotor and the small volume and high speed of the rotor of each one, then according to the different needs of job selection of other rotor.
Centrifugal angle rotor in the pipe can maintain a fixed angle centrifuge, can use the high speed centrifugal precipitation, slope is located in the bottom of the pipe. Large capacity of rotor can usually with different "adapter" to "fixed" small volume of sample tube, such as 6 x 85ml centrifugal rotor per hole can be placed 85ml tube, also can put 50ml or 15 20ml centrifuge tube, to achieve a multi-purpose; but because of the weight of the burden, the highest speed the rotor of the relatively small capacity is lower, so consider the highest centrifugal force can satisfy the experimental request. Because the speed of the rotor is usually adapted 2ml centrifugal refers to the tube of the rotor, sometimes a rotor price almost can buy a small centrifuge, also can reduce the loss of expensive equipment. There is a small desktop high-speed centrifuges also provides fixed angle rotor with different sizes (0.5ml and 2ml/1.5ml respectively with centrifugal tubes, in fact, unless it is often fixed samples using 0.5ml centrifuge tube and each centrifugal is relatively large, generally choose a 2ml/1.5ml rotor, with the adapter (for 0.2ml, 0.4ml, 0.5ml tube adapter) it can meet the needs of. If the commonly used PCR pipe should be considered with a rotor 8 Union more convenient.
The cover angle rotor with help to reduce friction, reduce noise and prevent accidents, the use of angle rotor must be covered, if not cover the centrifuge chamber will have a lot of eddy current resistance and frictional heating, which is equal to the increase of extra burden to the centrifuge motor and a refrigerating machine, centrifugal machine service life. In addition there is a tightness rotors can be airtight centrifugal, to prevent accidental leakage, is very important to improve the environment.
The level of the rotor is equipped with 2 or 4 baskets, stationary vertical hanging on the rotor, nacelle has a different adapter in order to meet the needs of different capacity of centrifugal tube. When the horizontal centrifugal tube rotor speed exceeds 600rpm, the sample deposition direction is along the tube axial movement, finally settling in the bottom of the pipe, convenient collection. Because as the fixed angle rotor stability, horizontal basket maximum speed compared to fixed angle rotor is much lower, limited to the scope of the low speed centrifugation. The basket has a different adapter, centrifugation can be placed on all types of pipe, and even meet the need of the flat plate centrifugal -- such as large capacity centrifuge Eppendorf can be equipped with multi-purpose adapter, so that a plurality of microplates or culture plates, deep hole board, PCR board, slide box, a large-scale nuclear acid purification kit purification plate, or cell culture bottle of centrifugation and large capacity centrifuge bottle public with a horizontal rotor, help to save money at the same time, to meet the diverse needs. So the better choice of rotor adaptation range is wide.
Horizontal rotor can make the centrifugal precipitation at the end of the pipe and non inclined wall, but the level of the rotor speed is often fixed rotor with models of the much slower -- sometimes can not meet the needs of high speed centrifugation, requirements for high speed but also requested the precipitation trace samples in the bottom of the pipe can choose drum type rotor, centrifugal to stent horizontal fixed not in this beautiful "circle biscuit box" inside, can at relatively high speed centrifugation (14000rmp) and ensure the precipitation gathered at the bottom of pipe.
More and more new rotor should be the new born of necessity. The rotor material from the light weight, high strength, aluminum alloy low cost continuous innovation, the carbon fiber rotor, rotor and so on ultra light plastic rotor, and the use of aluminum alloy mold pressed into hollow angle rotation so as to effectively reduce weight, so as to reduce the motor load and increase the capacity of the sample. Now most of the rotors and adapters, the cover can be directly high-temperature sterilization, corrosion resistance is greatly improved, can meet the special need. But the rotor at high speeds, produced inside the centrifugal stress, they have some use limit, need to refer to the instructions properly keep and use. When the centrifugal stress exceeds a certain limit, will cause the rotor premature damage, or even an accident, should strictly follow the manufacturer's instructions.